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mitochondrial complex iv activity  (Elabscience Biotechnology)


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    Elabscience Biotechnology mitochondrial complex iv activity
    LC attenuated myocardial <t>mitochondrial</t> structural damage and respiratory chain abnormalities in EAM mice. (A) Transmission electron microscopy showing morphological changes in mouse heart mitochondria (×5,000, scale bar = 2 μm). Changes in (B) mitochondrial complex IV activity and (C) ATP levels in each group, n = 3. (D,E) DCFH-DA staining flow cytometry to detect ROS levels in each group, n = 3. Data are expressed as Mean ± SEM with individual data points shown. ATP, adenosine triphosphate; ROS, reactive oxygen species.
    Mitochondrial Complex Iv Activity, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cytochrome+c+oxidase+activity+assay+kit/Cell+Mitochondrial+Complex+IV+(Cytochrome+C+Oxidase+)+Activity+Assay+Kit/pmc12827101-76-0-10
    Average 94 stars, based on 11 article reviews
    mitochondrial complex iv activity - by Bioz Stars, 2026-11
    94/100 stars

    Images

    1) Product Images from "Levocarnitine improves cardiac energy metabolic remodeling in myocarditis mice"

    Article Title: Levocarnitine improves cardiac energy metabolic remodeling in myocarditis mice

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2025.1706936

    LC attenuated myocardial mitochondrial structural damage and respiratory chain abnormalities in EAM mice. (A) Transmission electron microscopy showing morphological changes in mouse heart mitochondria (×5,000, scale bar = 2 μm). Changes in (B) mitochondrial complex IV activity and (C) ATP levels in each group, n = 3. (D,E) DCFH-DA staining flow cytometry to detect ROS levels in each group, n = 3. Data are expressed as Mean ± SEM with individual data points shown. ATP, adenosine triphosphate; ROS, reactive oxygen species.
    Figure Legend Snippet: LC attenuated myocardial mitochondrial structural damage and respiratory chain abnormalities in EAM mice. (A) Transmission electron microscopy showing morphological changes in mouse heart mitochondria (×5,000, scale bar = 2 μm). Changes in (B) mitochondrial complex IV activity and (C) ATP levels in each group, n = 3. (D,E) DCFH-DA staining flow cytometry to detect ROS levels in each group, n = 3. Data are expressed as Mean ± SEM with individual data points shown. ATP, adenosine triphosphate; ROS, reactive oxygen species.

    Techniques Used: Transmission Assay, Electron Microscopy, Activity Assay, Staining, Flow Cytometry

    LC improves myocardial mitochondrial substrate utilization in EAM mice. (A) Serum H-FABP levels in each group of mice, n = 3. (B–D) Western blot analysis of OCTN-2 and CPT-1B proteins, n = 3. (E) FFA and (F) LAC levels in myocardial tissue from each group of mice, n = 3. Data are expressed as Mean ± SEM with individual data points shown. FABP, heart-type fatty acid-binding protein; OCTN2, organic carnitine transporter novel type 2; CPT-1B, carnitine palmitoyltransferase-1B; FFA, free fatty acid; LAC, lactic acid.
    Figure Legend Snippet: LC improves myocardial mitochondrial substrate utilization in EAM mice. (A) Serum H-FABP levels in each group of mice, n = 3. (B–D) Western blot analysis of OCTN-2 and CPT-1B proteins, n = 3. (E) FFA and (F) LAC levels in myocardial tissue from each group of mice, n = 3. Data are expressed as Mean ± SEM with individual data points shown. FABP, heart-type fatty acid-binding protein; OCTN2, organic carnitine transporter novel type 2; CPT-1B, carnitine palmitoyltransferase-1B; FFA, free fatty acid; LAC, lactic acid.

    Techniques Used: Western Blot, Binding Assay

    Pearson’s correlation analysis of p-Akt and PGC-1α with mitochondrial metabolic function. Scatter plots showing the correlation between myocardial p-Akt protein expression and (A) COX IV activity, (B) CPT-1B expression, (C) ROS levels, and (D) ATP. Scatter plots showing the correlation between myocardial PGC-1α protein expression and (E) COX IV activity, (F) CPT-1B expression, (G) ROS levels, and (H) ATP. (n = 9 from 3 groups).
    Figure Legend Snippet: Pearson’s correlation analysis of p-Akt and PGC-1α with mitochondrial metabolic function. Scatter plots showing the correlation between myocardial p-Akt protein expression and (A) COX IV activity, (B) CPT-1B expression, (C) ROS levels, and (D) ATP. Scatter plots showing the correlation between myocardial PGC-1α protein expression and (E) COX IV activity, (F) CPT-1B expression, (G) ROS levels, and (H) ATP. (n = 9 from 3 groups).

    Techniques Used: Expressing, Activity Assay

    Related Articles

    Activity Assay:

    Article Title: MSCs-derived HGF alleviates senescence after AKI by modulating mitoSTAT3-controlled copper flux and respiration.
    Article Snippet: .. Measurement of respiratory chain complex IV activity After mPRTECs were lysed and centrifuged, Cytochrome C Oxidase Activity Assay Kit (E-BC-K837-M, Elabscience) was used according to the manufacturer’s instructions. .. Protein concentrations were determined by BCA (23225, Thermo, USA) in renal tissue directly or after cytoplasm and mitochondria isolation by extraction kit (C3601, Beyotime).

    Article Title: MSCs-derived HGF alleviates senescence after AKI by modulating mitoSTAT3-controlled copper flux and respiration
    Article Snippet: .. After mPRTECs were lysed and centrifuged, Cytochrome C Oxidase Activity Assay Kit (E-BC-K837-M, Elabscience) was used according to the manufacturer’s instructions. .. Protein concentrations were determined by BCA (23225, Thermo, USA) in renal tissue directly or after cytoplasm and mitochondria isolation by extraction kit (C3601, Beyotime).



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    Image Search Results


    LC attenuated myocardial mitochondrial structural damage and respiratory chain abnormalities in EAM mice. (A) Transmission electron microscopy showing morphological changes in mouse heart mitochondria (×5,000, scale bar = 2 μm). Changes in (B) mitochondrial complex IV activity and (C) ATP levels in each group, n = 3. (D,E) DCFH-DA staining flow cytometry to detect ROS levels in each group, n = 3. Data are expressed as Mean ± SEM with individual data points shown. ATP, adenosine triphosphate; ROS, reactive oxygen species.

    Journal: Frontiers in Pharmacology

    Article Title: Levocarnitine improves cardiac energy metabolic remodeling in myocarditis mice

    doi: 10.3389/fphar.2025.1706936

    Figure Lengend Snippet: LC attenuated myocardial mitochondrial structural damage and respiratory chain abnormalities in EAM mice. (A) Transmission electron microscopy showing morphological changes in mouse heart mitochondria (×5,000, scale bar = 2 μm). Changes in (B) mitochondrial complex IV activity and (C) ATP levels in each group, n = 3. (D,E) DCFH-DA staining flow cytometry to detect ROS levels in each group, n = 3. Data are expressed as Mean ± SEM with individual data points shown. ATP, adenosine triphosphate; ROS, reactive oxygen species.

    Article Snippet: Mitochondrial complex IV activity was measured with the kit (E-BC-K837-M, Elabscience, Wuhan, China) to assess the integrity of the mitochondrial respiratory chain.

    Techniques: Transmission Assay, Electron Microscopy, Activity Assay, Staining, Flow Cytometry

    LC improves myocardial mitochondrial substrate utilization in EAM mice. (A) Serum H-FABP levels in each group of mice, n = 3. (B–D) Western blot analysis of OCTN-2 and CPT-1B proteins, n = 3. (E) FFA and (F) LAC levels in myocardial tissue from each group of mice, n = 3. Data are expressed as Mean ± SEM with individual data points shown. FABP, heart-type fatty acid-binding protein; OCTN2, organic carnitine transporter novel type 2; CPT-1B, carnitine palmitoyltransferase-1B; FFA, free fatty acid; LAC, lactic acid.

    Journal: Frontiers in Pharmacology

    Article Title: Levocarnitine improves cardiac energy metabolic remodeling in myocarditis mice

    doi: 10.3389/fphar.2025.1706936

    Figure Lengend Snippet: LC improves myocardial mitochondrial substrate utilization in EAM mice. (A) Serum H-FABP levels in each group of mice, n = 3. (B–D) Western blot analysis of OCTN-2 and CPT-1B proteins, n = 3. (E) FFA and (F) LAC levels in myocardial tissue from each group of mice, n = 3. Data are expressed as Mean ± SEM with individual data points shown. FABP, heart-type fatty acid-binding protein; OCTN2, organic carnitine transporter novel type 2; CPT-1B, carnitine palmitoyltransferase-1B; FFA, free fatty acid; LAC, lactic acid.

    Article Snippet: Mitochondrial complex IV activity was measured with the kit (E-BC-K837-M, Elabscience, Wuhan, China) to assess the integrity of the mitochondrial respiratory chain.

    Techniques: Western Blot, Binding Assay

    Pearson’s correlation analysis of p-Akt and PGC-1α with mitochondrial metabolic function. Scatter plots showing the correlation between myocardial p-Akt protein expression and (A) COX IV activity, (B) CPT-1B expression, (C) ROS levels, and (D) ATP. Scatter plots showing the correlation between myocardial PGC-1α protein expression and (E) COX IV activity, (F) CPT-1B expression, (G) ROS levels, and (H) ATP. (n = 9 from 3 groups).

    Journal: Frontiers in Pharmacology

    Article Title: Levocarnitine improves cardiac energy metabolic remodeling in myocarditis mice

    doi: 10.3389/fphar.2025.1706936

    Figure Lengend Snippet: Pearson’s correlation analysis of p-Akt and PGC-1α with mitochondrial metabolic function. Scatter plots showing the correlation between myocardial p-Akt protein expression and (A) COX IV activity, (B) CPT-1B expression, (C) ROS levels, and (D) ATP. Scatter plots showing the correlation between myocardial PGC-1α protein expression and (E) COX IV activity, (F) CPT-1B expression, (G) ROS levels, and (H) ATP. (n = 9 from 3 groups).

    Article Snippet: Mitochondrial complex IV activity was measured with the kit (E-BC-K837-M, Elabscience, Wuhan, China) to assess the integrity of the mitochondrial respiratory chain.

    Techniques: Expressing, Activity Assay